Completely automated extraction of DNA from whole blood

Completely automated extraction of DNA from whole blood.

Labdisk Integrated Dna Extraction from Whole Blood Using Magnetic Particles

Integration of sample preparation is a key issue in the development of point of care diagnostic devices. We address the integration of a magnetic-particle based DNA extraction from 200 μl of whole blood into a centrifugal-microfluidic LabDisk. Quality of LabDisk extracted DNA was compared to DNA extracted by commercial spin columns as golden standard with four independent extractions from one b...

Direct PCR from whole blood, without DNA extraction.

Typically DNA used in PCR assays is usually extracted according to die phenol-chloroform method (1) or by an alternative 'saltingout' rapid purification (2). Moreover partially purified DNA obtained with rapid procedures have been reported to be suitable for PCR amplification (3). We describe here a more simple and efficient method to amplify DNA directly from whole blood samples without any pu...

Automated DNA Extraction from Plants

Tecan Journal 3/2003 4 www.tecan.com Research on plant diseases Downy mildew, caused by the oomycete Plasmopara viticola, is one of the most important grape (Vitis vinifera) diseases, which needs to be controlled by preventive fungicide application to reduce its erratic appearance and high reproduction potential. One of the research areas of the group of Cesare Gessler at the Institute of Plant...

Automated extraction and quantification of human cytomegalovirus DNA in whole blood by real-time PCR assay.

The measurement of human cytomegalovirus (HCMV) DNA in blood is becoming the standard method for monitoring HCMV infection in immune-suppressed and unsuppressed patients. As various blood compartments can be used, we have compared the HCMV DNA measured in whole blood (WB), peripheral blood leukocytes (PBL), and plasma by real-time PCR. We tested 286 samples: HCMV DNA was extracted automatically...